How does micellar electrokinetic chromatography work?
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In micellar electrokinetic capillary chromatography, surfactants are added to the buffer solution in concentration above their critical micellar concentrations, consequently micelles are formed; micelles that undergo electrophoretic migration like any other charged particle.
What is Meck micellar electrokinetic chromatography?
Micellar electrokinetic chromatography is a modified version of capillary electrophoresis which is used to separate a wide range of molecules, from inorganic ions to large biopolymers.
What is capillary chromatography?
Capillary electrochromatography (CEC) is a chromatographic technique in which the mobile phase is driven through the chromatographic bed by electroosmosis. Capillary electrochromatography is a combination of two analytical techniques, high-performance liquid chromatography and capillary electrophoresis.
How is MEKC detection done?
MEKC and CEC The column area is thermostated to maintain a constant temperature. A miniaturized optical detector positioned between the buffer reservoirs is commonly used for on-column detection. Some form of interlock mechanism is used to prevent operator exposure to the high voltages, up to 30–50 kV, typically used.
What is micellar electrokinetic capillary chromatography?
Micellar electrokinetic capillary chromatography, a hybrid method that combines chromatographic and electrophoretic separation principles, extends the applicability of capillary electrophoretic methods to neutral analytes.
What is capillary electrokinetic chromatography (CEC)?
Like MEKC, capillary electrokinetic chromatography (CEC) is a chromatographic technique performed with CE instrumentation. It employs fused silica capillaries packed with 1.5- to 5-μm microparticulate porous silica beads, usually derivatized with a hydrophobic ligand such as C18.
What are the different methods of cell electrophoresis?
The basic modes of CE that are presently being exploited include capillary zone electrophoresis (CZE), micellar electrokinetic chromatography (MEKC), capillary isotachophoresis, capillary isoelectric focusing, and capillary gel electrophoresis.
Why is micellar electrokinetic electrophoresis used to separate neutral and charged compounds?
Although micellar electrokinetic electrophoresis was initially used to separate neutral compounds, as it separates both neutral and charged compound with equal electrophoretic abilities, it is used for both now. The compounds in this method can be separated both on the basis of electrophoretic mobility and solute partitioning in the micelle.