WHAT is a BPG column?
Table of Contents
BPG columns are glass chromatography columns designed for industrial applications, which demand high standards of hygiene. The columns are constructed from component materials of the highest quality and withstand the harsh conditions used for cleaning in place of packed downstream purification resins.
What is a chromatography column made of?
Materials. Liquid chromatography: Traditional chromatography columns were made of glass. Modern columns are mostly made of borosilicate glass, acrylic glass or stainless steel. To prevent the stationary phase from leaking out of the column interior a polymer, stainless steel or ceramic net is usually applied.
How do I calculate column volume in HPLC?
PROCEDURE
- Use Volume = pi x radius2 x Length.
- pi = 3.14.
- r2 and Length should be converted to centimeters.
- Diameter of column divided by 2 = radius.
- radius x radius = r2
- 3.14 x r2 x L = Volume in cm3
- cm3 = 1 mL.
What are the different types of column chromatography?
Five chromatographic methods that use columns are gas chromatography (GC), liquid chromatography (LC), Ion exchange chromatography (IEC), size exclusion chromatography (SEC), and chiral chromatography. The basic principals of chromatography can be applied to all five methods.
What type of chromatography is column chromatography?
This method is a type of adsorption chromatography technique.
What size column do I need chromatography?
What size column do I need?
| Column Size (mL) | Fraction Size (mL) | Sample size |
|---|---|---|
| Separation | ||
| 2000 | 100 | 20-30 |
| 4000 | 150-200 | 50 |
| 8000 | 250-400 | 100 |
What type of column is used in HPLC?
HPLC columns are mostly four main types Normal phase, Reverse Phase, and Ion Exchange columns. In the pharmaceutical industry mostly reverse columns are used for analysis..
Why GC columns are longer than HPLC?
A gas encounters less resistance in the GC column which permits use of longer column lengths. Sample stability – samples analysed by HPLC are generally thermally labile so they are in liquid phase in the HPLC column at room temperatures.
What is the bed volume of a column?
The bed volume is defined as the minimum volume of solvent necessary to wet the defined quantity of sorbent within the. column.
What is an example of a column by Index?
For example, if you are renaming columns, and specify the columns by index, the number of names you supply must match the number of column indices. Another example might be a math operation that uses two columns, where the columns must have the same number of rows.
What are the dimensions of the columns?
The columns are simple to use and versatile in application. Scalable from inner diameters (i.d.) of 70 to 200 mm and bed volumes from 0.1 to 25 L.
What are the different types of index stands?
Stands and wheels: INdEX 70 and 100 stands have adjustable feet. Castors with brakes are available. INdEX 140 and 200 stands have castors with brakes as standard. Nets: The column is delivered with 23 µm (polypropylene) nets.
What are the different types of indexing?
1 Indexing is a small table which is consist of two columns. 2 Two main types of indexing methods are 1)Primary Indexing 2) Secondary Indexing. 3 Primary Index is an ordered file which is fixed length size with two fields. 4 The primary Indexing is also further divided into two types 1)Dense Index 2)Sparse Index.