What is osteogenic differentiation?
Table of Contents
Osteogenic Differentiation of Human/Mouse Mesenchymal Stem Cells. Mesenchymal stem cells (MSCs) are functionally defined by their capacity to self renew and their ability to differentiate into multiple cell types including adipocytes, chondrocytes, and osteocytes.
How do you improve osteogenic differentiation?
Mechanical vibrations, stress, and shear forces can all enhance osteogenic differentiation of MSCs while the lack of mechanical stimuli can induce adipogenesis [2,4]. Luu et al. have shown that low magnitude mechanical stimulation (LMMS) induces osteogenesis and inhibit adipogenesis [5,6].
Where is the osteogenic layer?
periosteum
Immature osteogenic cells are found in the deep layers of the periosteum and the marrow. They differentiate and develop into osteoblasts. The dynamic nature of bone means that new tissue is constantly formed, and old, injured, or unnecessary bone is dissolved for repair or for calcium release.
Do osteogenic cells undergo mitosis?
Osteogenic cells, however, can undergo mitosis. Osteogenic cells are cells that differentiate into osteoblasts. Once differentiated, the osteogenic cells can no longer undergo mitosis.
What is the difference between Osteoconduction and Osteoinduction?
Osteoinduction implies the recruitment of immature cells and the stimulation of these cells to develop into preosteoblasts. In a bone healing situation such as a fracture, the majority of bone healing is dependent on osteoinduction. Osteoconduction means that bone grows on a surface.
Do MSCs undergo distinct bioenergetic changes during osteogenic differentiation?
Based on these findings, we established that MSCs indeed undergo distinct bioenergetic changes during osteogenic differentiation, activating the mitochondrial process of OxPhos. Mitochondrial morphology in MSCs during osteogenic and adipogenic differentiation Mitochondria change morphology in accordance with the metabolic needs of the cell.
Is HIF-1α a potential regulator of energy metabolism during osteogenic differentiation?
Our data indicate that HIF-1α might serves as a possible regulator of MSC energy metabolism during osteogenic differentiation. It is most active in undifferentiated MSCs, but is dramatically downregulated in ost-MSC, where OxPhos is activated.
How does mitochondrial morphology change during osteogenic and adipogenic differentiation?
Mitochondrial morphology in MSCs during osteogenic and adipogenic differentiation Mitochondria change morphology in accordance with the metabolic needs of the cell. We assessed mitochondrial morphology using MTG, fluorescence microscopy, and EM.