What is TruSeq RNA-seq?
TruSeq Stranded Total RNA A robust, highly scalable whole-transcriptome analysis (RNA-Seq) solution for a variety of species and sample types, including human, mouse, and formalin-fixed, paraffin-embedded (FFPE) tissue.
What is Illumina TruSeq?
TruSeq DNA Library Preparation Kits are a simple, cost-effective solution for generating high-quality libraries, compatible with unparalleled Illumina sequencing output. TruSeq library preparation has become the industry’s most trusted and widely adopted library preparation method for next-generation sequencing (NGS).
What is RNA-seq workflow?
The general workflow of RNA-seq analysis. Quality control of raw reads. Quality control of RNA-seq raw reads consists of analysis of sequence quality, GC content, adaptor content, overrepresented k-mers, and duplicated reads, dedicated to detecting sequencing errors, contaminations, and PCR artifacts.
What is library preparation in RNA-seq?
Current RNA-seq library preparation methods comprise preparing rRNA-depleted or poly(A)-enriched RNA followed by RNA fragmentation, cDNA synthesis, adaptor ligation and multiple cleanup steps. These methods are generally time consuming, requiring about 1.5 d and significant hands-on time.
What is P5 and P7?
The P5 region is cleaved, resulting in clusters containing only fragments which are attached by the P7 region. This ensures that all copies are sequenced in the same direction. The sequencing primer anneals to the P5 end of the fragment, and begins the sequencing by synthesis process.
Is Illumina sequencing accurate?
Illumina sequencing chemistry delivers high accuracy, with a vast majority of bases scoring Q30 and above. This level of accuracy is ideal for a range of sequencing applications, including clinical research.
What is the difference between RNA-Seq and Qpcr?
While qRT-PCR is useful for quantifying the expression of a few genes, it can only detect known sequences. In contrast, RNA sequencing (RNA-Seq) using NGS can detect both known and novel transcripts.
What is the TruSeq™ small RNA sample preparation protocol?
The Illumina® TruSeq ™ Small RNA Sample Preparation protocol is used to prepare a variety of RNA species. The protocol takes advantage of the natural structure common to most known microRNA molecules.
What are the standard input protocols for RNA sequencing?
We used three standard input protocols: Illumina TruSeq Stranded Total RNA and mRNA kits, a modified NuGEN Ovation v2 kit, and the TaKaRa SMARTer Ultra Low RNA Kit v3.
What is TruSeq RNA exome?
TruSeq RNA Exome Provides a reproducible, economical solution for sequencing RNA from FFPE tissues and other low-quality samples. Accuracy from as little as 10 ng total RNA. TruSeq Stranded Total RNA with Ribo-Zero Globin
Which RNA-Seq library preparation protocol is best for protein-coding gene profiles?
At the manufacturers’ recommended input RNA levels, all the RNA-Seq library preparation protocols evaluated were suitable for distinguishing between experimental groups, and the TruSeq Stranded mRNA kit was universally applicable to studies focusing on protein-coding gene profiles. The TruSeq protoc …