What is PubMLST?
Table of Contents
PubMLST – Public databases for molecular typing and microbial genome diversity.
What is BIGSdb?
BIGSdb is software designed to store and analyse sequence data for bacterial isolates.
How is MLST performed?
The principle of MLST is simple: the technique involves PCR amplification followed by DNA sequencing. Nucleotide differences between strains can be checked at a variable number of genes depending on the degree of discrimination desired.
Who invented MLST?
MLST technique was first used by Maiden et al. (1) to characterize Neisseria meningitidis using six loci. The application of MLST has clearly resolved the major meningococcal lineages known to be responsible for invasive disease around the world.
How is MLST done?
The workflow of MLST involves: 1) data collection, 2) data analysis and 3) multilocus sequence analysis. In the final analysis step of MLST, the relatedness of isolates are made by comparing allelic profiles. Researchers do epidemiological and phylogenetical studies by comparing STs of different clonal complexes.
Why is MLST important?
MLST is a powerful tool for understanding the evolutionary dynamics of pathogens and to gain insight into their genetic diversity. We illustrate the importance of accurate typing by reporting on three problems that have arisen in the study of a single bacterial species, the plant pathogen Xylella fastidiosa.
How do you do an MLST analysis?
The workflow of MLST involves: 1) data collection, 2) data analysis and 3) multilocus sequence analysis. In the data collection step, definitive identification of variation is obtained by nucleotide sequence determination of gene fragments.
What is the pubmlst database?
The PubMLST.orgwebsite hosts a collection of open-access, curated databases that integrate population sequence data with provenance and phenotype information for over 100 different microbial species and genera.
What is the pubmlst molecular typing database?
The PubMLST molecular typing databases, powered by the Bacterial Isolate Genome Sequence Database (BIGSDB) are scalable, web-accessible databases that meet these needs. BIGSDB enables phenotype and sequence data, which can range from a single sequence read to whole genome data, to be efficiently linked to a limitless number of bacterial specimens.
What is pubmlst and why should I Care?
“PubMLST hosts a collection of curated databases that integrate population sequence data with provenance and phenotype information for over 100 different microbial species and genera.”
Why pubmlst for bacterial population genomics?
The opportunities for bacterial population genomics that are being realised by the application of next-generation sequencing require novel bioinformatics platforms. The PubMLST molecular typing databases, powered by the Bacterial Isolate Genome Sequence Database (BIGSDB) are scalable, web-accessible databases that meet these needs.