What are some advantages of ISE?
ISE has many advantages compared to other techniques, including:
- It is relatively inexpensive and easy to operate.
- It has wide concentration measurement range.
- As it measure the activity, instead of concentration, it is particularly useful in biological/medical application.
What is application of ion-selective electrode in clinical laboratory science?
Ion-selective electrodes are used to determine the activity of the ion under consideration (analyte ion). The ion has to be dissolved preferably in water. When the sample does not dissolve, the ion can also be extracted. Otherwise, color and turbidity of a sample do not disturb the measurement.
How do ion electrodes work?
Ion selective electrodes allow us to measure the activity of a specific ion in a sample, by measuring the potential of a measuring cell which has a membrane that is specific to a certain ion, against the potential of a reference cell.
What is ISE in clinical chemistry?
ISE means ion-selective electrodes. Indirect ISE: Measures on a total plasma sample (or serum) that has been diluted with a large volume of diluent. Requires that the plasma and erythrocytes are separated by centrifugation.
What is the advantage of potentiometry in medical technology?
It is worth standing and mentioning that potentiometric technique is highly useful for the estimation of simple ions, particularly for pH and physiological electrolytes, more likely their concentration which is wholly and sole importance from the biomedical point of view.
Why are electrodes important in potentiometry?
In a potentiometric measurement, an indicator electrode responds to changes in the activity, or “effective concentration” of the analyte. A potential, or voltage, that develops at the interface between the electrode and the analyte solution is measured relative to a reference electrode.
What is the role of working electrode in potentiometry?
A potentiostat requires an electrochemical cell with three electrodes. The Working Electrode is the electrode where the potential is controlled and where the current is measured. For many physical electrochemistry experiments, the Working Electrode is an “inert” material such as gold, platinum, or glassy carbon.
What are the applications of electrodes in everyday life?
The potential of these electrodes in solving many practical problems is now being realized. For example, they are widely used in clinical, biological, water, air, oceanographic, and pharmaceutical research and in routine analytical determinations.
What are ion-selective electrodes?
Ion-selective electrodes (ISEs) are the oldest class of chemical sensors and they are still superior to other sensor types used in various applications in biomedical, industrial, and environmental fields.
What is a chloride electrode used for?
The chloride electrode has been used for the determination of chloride ion in a variety of industrial and physiological samples. Of particular importance is the rapid, accurate clinical determination of chloride ion in sweat.
Can potentiometric microelectrodes be used for in situ analysis?
However, in situ analysis requires a much smaller electrode that can be inserted directly into a cell. For this purpose, potentiometric microelectrodes have been developed for a number of clinically important analytes, including hydrogen, potassium, sodium, calcium and chloride ions.