What is pBluescript plasmid?
Table of Contents
The pBluescript II phagemids (plasmids with a phage origin) are cloning vectors designed to simplify commonly used cloning and sequencing procedures, including the construction of nested deletions for DNA sequencing, generation of RNA transcripts in vitro and site-specific mutagenesis and gene mapping.
Is pBluescript a phagemid?
The pBluescript vectors, e.g., pBluescript II KS/SK(+ / –) (3 kb), are phagemids derived from pUC19 and have essentially similar configurations as pGEM-Zf except that they have T7 and T3 promoters, respectively, flanking the MCS which is composed of 21 unique restriction sites (95).
What is the size of pBluescript?
Plasmid: pBluescript SK (+)
| Source/Vendor: | Stratagene |
|---|---|
| Plasmid Type: | Bacterial Expression |
| Cloning Method: | Restriction Enzyme |
| Size: | 2958 |
| 5′ Sequencing 1 Primer: | M13pUC-fwd |
What is polylinker sequence?
A polylinker is a short DNA sequence containing two or more different sites for cleavage by restriction enzymes. Polylinkers are introduced into vectors to make cloning easier by providing sites that allow cloning DNA, cut with any of a number of different restriction enzymes, into a single plasmid.
What is f1 origin?
The ori is the place where DNA replication begins, enabling a plasmid to reproduce itself as it must to survive within cells (Addgene). F1 is a phage-derived ori that allows for the replication and packaging of ssDNA into phage particles. Plasmids with phage-derived ori’s are referred to as phagemids.
What is pBluescript SK+?
The pBluescript II SK+ phagemid (plasmids with a phage origin) is a cloning vectors designed to simplify commonly used cloning and sequencing procedures, including the construction of nested deletions for DNA sequencing, generation of RNA transcripts in vitro and site-specific mutagenesis and gene mapping.
Is pUC18 and expression vector?
These vectors provide novel cloning possibilities in addition to simple, non-expensive, high level expression of recombinant proteins in E. coli. Vectors pUC18 and pUC19 are small high-copy number plasmids that are widely used for cloning and manipulation of DNA fragments (9).
Is the pBluescript KS vector available from Addgene?
This vector is NOT available from Addgene. Standard cloning vector (phagemid excised from lambda ZAP). The f1 (+) orientation allows rescue of sense strand ssDNA. pBluescript KS (+) and pBluescript SK (+) differ by the orientation of the MCS.
What is the orientation of the pBluescript SK+ vector?
Standard cloning vector (phagemid excised from lambda ZAP). The f1 (+) orientation allows rescue of sense strand ssDNA. pBluescript SK (+) has a reversed MCS.
What are pBluescript II phagemids?
INTRODUCTION The pBluescript II phagemids (plasmids with a phage origin) are cloning vectors designed to simplify commonly used cloning and sequencing procedures, including the construction of nested deletions for DNA sequencing, generation of RNA transcripts in vitro and site-specific mutagenesis and gene mapping.
How is pBluescript SK excised from a lambda phage?
In E. coli a phagemid, pBluescript SK (−), contained within the vector, can be excised by 11 or M13 helper phage. The excision process elimlnates the need to subclone DNA inserts from the lambda phage into a plasmid by restriction digestion and ligation.