How do you dilute a 1kb ladder?

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If you want to prepare a working stock of the 1 kb ladder with loading dye, measure amount of ladder in the tube and add the loading dye accordingly. Use water to dilute your loading dye, in the ratio of 1 part ladder, 1 part buffer, and 4 parts ddH20 (if your loading buffer is 6X).

How do you make a 100 bp DNA Ladder?

This ladder is preferred by our lab because of its broad range (100 bp to 10,000 bp) and even intervals. Its stock concentration is 1 µg/µl of DNA. The ladder is diluted to a 1:4 solution in water for use (3 parts water for 1 part ladder). To make 100 µl, 75 µl of water are combined with 25 µl of the DNA ladder.

Can I dilute DNA Ladder?

Dilute only 1 µl of DNA Ladder at a time DNA may denature if diluted and stored in dH20. This protocol is recommended for a 5mm wide gel lane. The components of the mixture should be scaled up or down, depending on the width of the lane.

How do you dilute a ladder?

The ladder is diluted to a 1:4 solution in water for use (3 parts water for 1 part ladder). To make 100 µl, 75 µl of water are combined with 25 µl of the DNA ladder.

What is the concentration of 1KB DNA ladder?

The concentration of 1kb DNA Ladder is 100 ug/ml (500uL ) Five microlitres (500 ng) of the 1 kb DNA Ladder are mixed with 1uL of blue/orange 6X loading dye. I am using this Ladder in gel electrophoresis to see the amplified DNA band in after PCR.

What is the recommended load for electrophoresis using 1 KB ladder?

1 kb ladder can be bought commercially at various concentrations, but the recommended load for an electrophoresis run is 0.5 µg (5µl). These can be used in either agarose or in polyacrylamide gels with the concentration of gel at 0.75% to 1%. These ladders come with different tracking dyes like bromophenol blue, xylene cyanol FF.

What is Invitrogen DNA ladder 1kb plus?

The Invitrogen™1Kb Plus DNA Ladder is designed for sizing and quantification of double stranded DNA on 0.7% to 1.2% agarose gels. The 1 Kb Plus DNA Ladder consists of 18 individual chromatography-purified DNA fragments ranging in size from 100 bp to 15,000 bp.